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College of Veterinary & Animal Sciences , Mannuthy

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Departments

DEPARTMENT: Veterinary Physiology

Faculty & Staff

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Dr.Raji Kanakkaparambil Dr.Raji Kanakkaparambil
Designation: - Assistant Professor
Address: - Department of Veterinary Physiology, CVAS Mannuthy
Mobile No: - 9747237259
Email ID: - raji@kvasu.ac.in

Educational Qualifications

1. Post- Doc. (2016) - School of Women's & Children's Health, University of New South Wales, Sydney, Australia 

 

2. Ph.D.(2008)- University of Nottingham, School of Biosciences,Sutton Bonington,  UK 

 

3. P. G. Diploma in Bioinformatics (2004)- Bharathiar University, Coimbatore, Tamilnadu, India 

 

4.  M.V.Sc.in Animal Physiology(1997) - College of Veterinary and Animal Sciences, Kerala Agricultural University, Thrissur, Kerala, India 

 

5. B. V. Sc. & A.H.(1994) - College of Veterinary and Animal Sciences, Kerala Agricultural University, Thrissur, Kerala, India

   

Area of Research

Molecular Reproductive Physiology, Oocyte Biology and Stem Cell Biology

For Ph. D thesis project, as a part of NIH research programme to investigate the effects of altering methyl cycle metabolism by dietary means on DNA methylation in oocytes in University of Nottingham, I have been studying the effect of methyl deficient diet on epigenetic changes and expression of certain genes involved in ovarian follicular development in sheep. During Post-doctoral research in UNSW, Australia, I have been working on epigenetic changes in mouse oocytes due to pre- in vitro maturation (Pre- IVM) and in vitro maturation (IVM) treatments.I have under gone 3 months training in the area of stem cell research  in Bioinformatics Institute (BII), and Institute of Medical Biology(IMB),  A*STAR, Singapore. There, I did get an opportunity to work with both mesenchymal stem cells and embryonic stem cells.

RESEARCH PROJECTS

Completed Research Projects: As Principal Investigator

Study of expression of Prolificacy genes controlling follicular development in Malabari goats : State Plan fund : 10 Lakhs

Proteome analysis of goat ovarian follicles during different developmental stages: State Plan fund: 5 Lakhs

Immuno-histochemical localization of Transforming Growth Factor β (TGF-β) superfamily members in developing follicles of Malabari goats- State Plan Fund -10 Lakhs

On-going Research Project as Principal Investigator

Ascertaining a reliable method for isolation and purification of mesenchymal stem cells from dogs- State Plan Project- 5 lakhs

Completed Research Project: As Co PI

Ovsynch programme and its modifications on the reproductive performance of anoestrous and repeat breeding cattle. – Animal Husbandry Department, Kerala- 10 lakhs

Preliminary studies on capture myopathy and its control in various deer species of Kerala.-State Plan Fund 2012-13 – 3.5lakhs

Study of behavioural pattern and evaluation of stress in free ranging Asian elephants (Elephas maximus) involved in human-elephant conflict in Kerala.- State Plan Fund 2012-13 – 3.5lakhs

Comparative histological and Immuno histochemical studies on the pancreas of Malabari and Attappady Black breeds of goats.- State Plan fund- 2017-17- 5 lakhs

Identification of the Causative genes and Mutations Conferring Resistance against Tick Infestation in Cattle- Kerala State Council for Science, Technology and Environment- 37.49lakhs

 

TECHNICAL EXPERTISE

  • Primary cell culture
  • Maintenance and preservation of cell lines
  • PCR & Real time PCR Techniques
  • Protein extraction & SDS -PAGE
  • Western blotting
  • 2D gel electrophoresis
  • Immunohistochemistry and Immunofluorescence studies of tissue sections
  • Pre-IVM and IVM of mouse oocytes
  • Confocal microscopy
  • Microarray and its data analysis using Partek software
  • Isolation, culturing, characterization and differentiation of embryonic and mesenchymal stem cells.
  • Flow Cytometry
  • RIA, ELISA
  • Working knowledge of HPLC, GCMS
  • Other basic biotechnological, and biochemical techniques

Work Experience

2011- till date - Assistant Professor, Kerala Veterinary and Animal Sciences University, Kerala, India

2004- 2011 - Assistant Professor, Kerala Agricultural University, Kerala, India

1996- 2004 - Veterinary Surgeon, Department of Animal Husbandry, Kerala, India

Awards

  1. Endeavour Research fellowship for pursuing Post-Doctorate from University of New South Wales, Australia
  2. Visiting Scientists Fellowship- Scientific and Technological Research Council of Turkey (TÜB?TAK)
  3. International training in the area of stem cell research (Animal Sciences) at Bioinformatics Institute (BII) and Institute of Medical Biology (IMB),  A*Star, Singapore - sponsored by NAIP,  Indian Council of Agricultural Research
  4. Foundation grant for the final year of PhD from British Foundation for Women Graduates (BFWG), UK.
  5. Overseas Research Students Award (ORSAS) for pursuing PhD from UK          
  6. Full tuition fee scholarship of University of Nottingham, UK for pursuing PhD 
  7. University Ist Rank for MVSc

8. Indian Council of Agricultural Research (ICAR) Junior Fellowship for MVSc

  1. University Ist Rank for BVSc & AH
  2. Trivandrum Livestock Improvement Association Prize awarded by Kerala Agricultural University for securing the highest grade point average in the BVSc & AH
  3. Sri. T.V. Kochu Vareed Memorial Prize awarded by Kerala Agricultural University for highest grade point in BVSc & AH.
  4. Sri. M.O. Thomakutty Memorial Prize awarded by Kerala Agricultural University for securing the highest grade point in the group of courses of Biochemistry and Physiology.
  5. Dr. K S Nair Memorial Prize awarded by Kerala Agricultural University for securing highest grade point average in Animal Breeding and Genetics, Nutrition, Livestock Production Management and Dairy Science
  6. Inauguration Souvenir Prize awarded by Kerala Agricultural University for securing the highest-grade point average in the group of courses of Microbiology, Parasitology and Pathology.
  7. VIII Dairy Industry Conference Prize awarded by Kerala Agricultural University for the highest grade point average in    Livestock production Management, Dairy Science, Farm training and nutrition.

Trainings

  • A Short Course on “Recent Advances in Stem Cell Research” organized by Division of Physiology & Climatology, IVRI, Izatnagar and funded by Indian Council of Agricultural Research from 15-2-2010 to 7-3-2010
  • International Training on “Stem Cell Research (Animal Sciences)” funded by National Agricultural Innovation Project,-Component -4- ICAR from 18th May to 15th August 2011 at Bioinformatics Institute, A*STAR, Singapore and Institute of Medical Biology, A*STAR, Singapore
  • National Training programme on “Computational Aspect for NGS Data Analysis: A Sojourn from Lab to Field” at Ome Research Facility, Anand Agricultural University, Anand during March 04-13, 2014 under NAIP funded project Establishment of National Agricultural Bioinformatics Grid in ICAR
  • Summer School on “Improving Reproduction Rate through Assisted Reproductive and Stem cell Technologies for enhancing production in Small Ruminants” from July 6 to 26, 2017 at CIRG, Mathura sponsored by Education Division, Indian Council of Agricultural Research, New Delhi
  • Training Programme on "Advanced Bioinformatics tools and its Applications in Agriculture” from September 25-29, 2018 at NAARM, Hyderabad sponsored by Indian Council of Agricultural Research, New Delhi.

Publications

PAPERS PUBLISHED

Abhina Mohanan, Bhuvana Plakkot, Raji Kanakkaparambil (2019). Biochemical constituents and steroid hormones in follicular fluid from antral follicles of cross bred Malabari goats of Kerala. Biological Rhythm Research. DOI: 10.1080/09291016.2018.1564574.

Abhina Mohanan, Bhuvana Plakkot and Raji Kanakkaparambil (2018). Exprssion profile of BMPRIB gene in developing ovarian follicles of cross bred Malabari goats. The Pharma Innovation Journal. 7(12): 363-367.

Bhuvana Plakkot, Shiji Sheeja Saju and Raji Kanakkaparambil (2018). A review article on gonadotropins and their significant contribution in ovarian follicl development. The Pharma Innovation Journal. 7(11): 433-438.

Abhina Mohanan, Raji Kanakkaparambil (2018). Role of diet in epigenetics: a review. Journal of Dairy, Veterinary and Animal Research. 7(3):129-131 

Anu J. P., C. T. Sathyan, R. Geetha, K. Radha, K. Raji and Gleeja V. L. (2017). A study on impact of somatic cell count on physicochemical properties of pooled milk.  J. Vet. Anim. Sci.. 48 (2): 13-15

M. K. Aliciah, J. Metilda, M. O. Kurian, M. P. Unnikrishnan and K. Raji (2017). Morphological survivability of immature and mature bovine oocytes vitrified by cryoloop method. J. Vet. Anim. Sci.  48(1):1-5

G. Girish Varma and K. Raji (2017). Subtle adaptive biochemical mechanisms linking nutrition and reproduction at higher forms of life- A comprehensive perspective. In abstracts and souvenir of second annual convention of society of veterinary biochemists and biotechnologists of India and National symposium on Innovative and emerging biochemical and biotechnological tools to augment animal health and production. pp. 6-16

Aziz Zarina, G. G. Varma, K. Karthiayini, K. Raji And V. Beena (2017). Effect of varying temperature humidity index on the plasma volume of crossbred cattle calves. Journal of Agrometeorology. 19: 120-122

Aziz Z., Varma, G. G.,  Raji K., Beena, V. and Anil., K. S. (2016). Haematological responses of murrah buffalo calves to varying temperature humidity index. International Journal of Innovative Research and Development. 5(5):329- 331

Z. Aziz, G. G. Varma, K. Raji, and K. Karthiayini. (2016). Physiological response of murrah buffalo calves to varying temperature humidity index prevalent in central Kerala. J. Vet. Anim. Sci. 47(2): 21-24

Raji Kanakkaparambil and Kevin D Sinclair (2015). Effect of different doses of FSH on steroidogenic capability, proliferation and differentiation of sheep granulosa cells. J. Vet. Anim. Sci. 46(1): 5-10

Sneha S. Panicker, Raji Kanakkaparambil, Promod Kanjirakuzhiyil, and Ramachandran Koodathil (2015). Progesterone Profile of Goats Subjected to Oestrus Synchronization and its Relevance in Predicting Pregnancy Outcome. Adv. Anim. Vet. Sci. 3(4): 207-210.

Sneha Surendra Panicker, Promod Kanjirakuzhiyil, Ramachandran Koodathil, and Raji Kanakkaparambil (2015). Oestrous response and conception rate in Malabari cross-bred goats following two different oestrous synchronization protocols. J. Anim. Health Prod. 3 (2): 39 -42

Prashant U., Shynu M., Raji K., Pellissery A.J., Jayavardanan K.K. and George S. Sexing of monomorphic birds by amplification of chd 1 gene (2014).  Proceedings of the 26th Kerala Science Congress, Pookode, Wayanad, 28-31st January 2014; 652-654

Aparna M., Vimalkumar M.B., Varghese S., Senthilvel K., Ajithkumar K.G., Raji K., Syamala K., Priya M.N., Deepa C.K., Jyothimol G., Juliet S., Chandrasekhar L and Ravindran R. (2013). Phylogenetic analysis of bovine Theileria spp. isolated in south India. Tropical Biomedicine 30(2): 281–290

Raji Kanakkaparambil., Ravinder Singh., Dongfang Li., Robert Webb and Kevin D Sinclair (2009): B-Vitamin and Homocysteine Status Determines Ovarian Response to Gonadotropin Treatment in Sheep. Biol. Reprod. 80(4): 743–752.

Raji K., Surendranathan K.P and Philomina P.T. (2000): Effect of age and sex on the blood sugar profile in Japanese quail (Coturnix coturnix japonica). J. Vet. Anim. Sci.31

Raji K., Surendranathan K.P and Philomina P.T. (2000): Correlation of blood glucose and liver glycogen profile of Japanese quail at different stages of growth. J. Vet. Anim.Sci.31:47-52

Raji K., Surendranathan K.P. and Philomina P.T. (1999): Effect of feed restriction on body weight gain, liver weight and the levels of blood glucose and liver glycogen in Japanese quails. Indian J .Poult. Sci. 34(2):201-206

Raji K., Surendranathan K.P. and Philomina P.T. (1999). Correlation between the body weight and liver weight of Japanese quails from the day of hatch to sixteen weeks of age. Indian J. Poult.Sci. 34(2):251-254

Accepted for publication

S. Harikumar, K. S. Anil, Joseph Mathew, A. Prasad, K. A. Mercey, K. Ally and K. Raji. (2018). Continous measurement of core body temperature of dairy cows in winter and summer using Thermochron i button. Journal of Veterinary and Animal Sciences. Accepted for publication.

S. Harikumar, K. S. Anil, Joseph Mathew, A. Prasad, K. A. Mercey, K. Ally and K. Raji. (2018). Behavioural pattern of dairy cows under different therml stress management systems in summer. Journal of Veterinary and Animal Sciences.  Accepted for publication.

 

PAPERS PRESENTED

Invited Presentation

G. Girish Varma and K. Raji (2017). Subtle adaptive biochemical mechanisms linking nutrition and reproduction at higher forms of life- A comprehensive perspective. Lead paper presented in second annual convention of society of veterinary biochemists and biotechnologists of India and National symposium on Innovative and emerging biochemical and biotechnological tools to augment animal health and production.2-3rd June 2017, Veterinary College, Hebbal, Bangalore, India

Scientific Presentations

Anjana A, K. Raji and T. V. Aravindakshan (2018). Isolation of theca cells from goat ovarian follicles and expression of CYP19 gene in the isolated cells. Asian Regional Conference on Goats, ARCG 2018

Reshmi Sasi, Raji K. and Aravindakshan T. V. (2018). Polymorphism studies of sheep fecundity genes in Malabari and Attappady Black goat breeds of Kerala. International Confernce on Recent Trends in Environment, Sustainabilit, Agriculture and Life Sciences, 2nd and 3rd July, 2018

Abhina Mohanan and Raji Kanakkaparambil (2017).  Comparative expression profile of major prolificacy genes in ovarian follicles at various developmental stages in goat breeds of Kerala. NCRM- NICHE, 23rd October 2017 in Tokyo, Japan.

Dulama Richani, Sonia Bustamante, Cathy F. Lavea , Raji Kanakkaparambil , Nady Braidy , David J. Agapiou, Michael J. Bertoldo, Robert B. Gilchrist. (2017). Cyclic AMP modulated-IVM differentially impacts oocyte and cumulus cell metabolism. World Congress of Reproductive Biology, September 27-29,  2017, Okinawa, Japan.

Abhina M., Nikhil Kumar Tej, Amitha Thomas and Raji Kanakkaparambil. (2016). Biochemical constituents and steroid hormones in follicular fluid of Malabari goat. iCOMOS 2016, University of Minnesota, USA, April 24- 27, 2016

Abhina M., Nikhil Kumar Tej, Upasana R., Venkitachalapathy Tirupathi and Raji K. (2015). Expression profile of BMPR1B gene in ovarian follicles at various developmental stages and steroid hormone levels in two goat breeds of Kerala. In XXXIth Annual  Convention  and National Symposium on Current Challenges and Opportunities in Animal Reproduction organized by Indian Society for the Study of Animal Reproduction (ISSAR) from 3rd to 5th December 2015 at Veterinary College, Bangaluru, India.

R. S. Abhilash, K. Anil Kumar, K. Raji and Metilda Joseph. (2015). Early Pregnancy Diagnosis in Bovines- A comparative study between prostaglandin induced milk flow test (PG-IMFT) and progesterone assay. In XXXIth Annual  Convention  and National Symposium on Current Challenges and Opportunities in Animal Reproduction organized by Indian Society for the Study of Animal Reproduction (ISSAR) from 3rd to 5th December 2015 in Veterinary College, Bangaluru.

Abhina M., Raji K., and Aravindakshan T. V. (2015). Expression profile of bmpr1b gene in developing ovarian follicles of cross-bred Malabari breed of goats in Kerala. XXIV Annual conference of SAPI & National Symposium, Guwahati. January 2016.

 

INVITED TALKS

Stem cell techniques: Given to Faculty of Veterinary Medicine, University of Ankara, Turkey

Prolificacy studies of goats in India: Given Livestock Central Research Institute, Ankara, Turkey

Stem cell techniques: Given to Livestock Central Research Institute, Ankara, Turkey

Basic Concepts of Epigenetics: Presentation given in training programme conducted by School of Applied Animal Production and Biotechnology, Kerala Veterinary and Animal Sciences University, Kerala


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