Faculty

MVSc, PhD

1. Conservation Genetics

2. Genomics and Bioinformatics

3. Host-Pathogen Interactions

Assistant Professor (April 2015-Continuing)

1. ICAR sponsored winter school training programme titled “Recent advancement in recording, analysis and interpretation of novel Phenomics of livestock” at Division of Animal Genetics, Indian Veterinary Research Institute, Bareilly from 16 November to 06 December 2018.
2. MHRD sponsored induction training programme at the Department of Education, University of Calicut, India, from 02 May to 31 May 2018. 
3. ICAR sponsored advanced faculty training programme titled “Application of OMICS tools and techniques for Agricultural Germplasm Improvement” conducted at Centre for Agricultural Bioinformatics (CABin), Indian Agricultural Statistics Research Institute, New Delhi, India from 09 February to 01 March 2018.
4. Training programme on “Basic Course on Use of Bioinformatics Tools in Veterinary Science Research” at Bioinformatics Centre, Indian Veterinary Research Institute, Bareilly, from 13^th October to 15^th October 2014.
5. Training programme on “Phylogenetic Analysis: Techniques and Tools” at the Department of Biotechnology and Bioinformatics Centre, Barkatullah University, Bhopal, from 17^th January to 21^st January 2012.

JOURNAL ARTICLES

• Desai, A.G., Naicy, T., Aravindakshan, T.V., Asaf, V.N.M., Bindu, L., Bhat, V. and Haritha, B.P. (2019). Relative abundance of early growth response 2 (EGR2) mRNA in reproductive tissues of malabari goats. Ruminant Science (Accepted for publication).
• Sulabh, S., Panigrahi, M., Varshney, R., Gupta, J.P., Kumar, S., Verma, A., Kumar, A., Asaf, M., Kumar, P. and Bhushan, B. (2019). In-vitro analysis of Interleukin-10 expression in cell cultures of Crossbred cattle, Tharparkar cattle and Murrah buffalo in response to mastitis causing antigens derived from Staphylococcus aureus and Escherichia coli. Biol Rhythm Res, doi:10.1080/09291016.2019.1628407
• Suraj, S.V., Nair, S.N., Juliet, S., Sanjay, B.M., Saranya, C., Asaf, M., Shijin, M.S., Nisha, A.R. and Anoopraj, R. (2019). Single dose oral pharmacokinetics of sparfloxacin in rats. The Pharma Innovation J, 8(12):317-322.
• Gupta, J.P., Bhushan, B., Asaf, V.N.M., Kumar, A., Ranjan, S., Panigrahi, M., Kumar, A. and Kumar, P. (2018). Association and expression analysis of single nucleotide polymorphisms of CD14 gene with somatic cell score in crossbred cattle. Gene Rep, 12:255-260. doi:10.1016/j.genrep.2018.07.011
• Ranjan, S., Bhushan, B., Gupta, J.P., Asaf, V.N.M., Panigrahi, M., Kumar, A. and Raveendran, R. (2017). Study on genetic variation of microsatellite and their association with mastitis occurrence in crossbred cattle. J Anim Res, 7(1):127-132. doi:10.5958/2277-940X.2017.00018.3
• Kumar, A., Gaur, G.K., Gandham, R.K., Panigrahi, M., Ghosh, S., Saravanan, B.C., Bhushan, B., Tiwari, A.K., Sulabh,S.,  Priya, B., Asaf V.N.M., Gupta, J.P., Wani, S.A., Sahu, A.R. and Sahoo, A.P. (2017). Global gene expression profile of peripheral blood mononuclear cells challenged with theileria annulata in crossbred and indigenous cattle. Infect Genet Evol, 47:9-18.  doi:10.1016/j.meegid.2016.11.009
• Gupta, J.P., Bhushan, B., Panigrahi, M., Ranjan, S., Asaf, V.N.M., Kumar, A. and Gaur, G.K.  (2016). Effect of non-genetic factors on somatic cell measures in Vrindavani cattle. Indian J Dairy Sci, 69(1):128-131.
• Gupta, J.P., Bhushan, B., Panigrahi, M., Ranjan, S., Asaf, V.N.M., Kumar, A., Sulabh, S., Kumar, A., Kumar, P. and Sharma, D. (2016). Study on genetic variation of Short Tandem Repeats (STR) markers and their association with Somatic Cell Scores (SCS) in crossbred cows. Indian J Anim Res, 50(4):450-454. doi:10.18805/ijar.6709
• Asaf, V.N.M., Kumar, A., Raut, A.A., Bhatia, S. and Mishra, A. (2015). In-silico search of virus-specific host microRNAs regulating avian influenza virus NS1 expression. Theor Biosci, 134:65-73. doi:10.1007/s12064-015-0211-9
• Dewangan, P., Panigrahi, M., Kumar, A., Saravanan, B.C., Ghosh, S., Asaf, V.N.M., Parida, S., Gaur, G.K., Sharma, D. and Bhushan, B. (2015).  The mRNA expression of immune-related genes in crossbred and tharparkar cattle in response to in vitro infection with theileria annulata. Mol Biol Rep, 42(8):1247-1255. doi:10.1007/s11033-015-3865-y
• Asaf, V.N.M., Bhushan, B., Panigrahi, M., Kumar, A., Dewangan, P., Gaur, G.K. and Sharma, D. (2015). Lack of association of allelic variants of BRCA1 gene with mastitis susceptibility in Vrindavani cattle. Indian J Anim Sci, 85(1):81-83.
• Kumar, A., Panigrahi, M., Bhushan, B., Sulabh, S., Asaf V.N.M., Gupta, J.P., Saravanan, B.C., Ghosh, S. and Gaur, G.K. (2015). Expression profile of CXCL3 gene in peripheral blood mononuclear cells challenged in vitro with theileria annulata in crossbred cattle. J Anim Res, 5(2):373-375. doi:10.5958/2277-940X.2015.00063.7
• Asaf, V.N.M., Kumar, A., Panigrahi, M., Dewangan, P., Gaur, G.K. and Bhushan, B. (2014). Association of single nucleotide polymorphism in the MBL1 gene with mastitis in vrindavani crossbred cattle. Indian J Anim Sci,  84(6):703-705.
• Asaf, V.N.M., Bhushan, B., Panigrahi, M., Dewangan, P., Kumar, A., Kumar, P. and Gaur, G.K. (2014). Association study of genetic variants at single nucleotide polymorphism rs109231409 of mannose-binding lectins 1 gene with mastitis susceptibility in vrindavani crossbred cattle. Vet World, 7(10):807-810.
• Asaf, V.N.M., Kumar, A., Rahim, A., Sebastian, R., Mohan, V., Dewangan, P. and Panigrahi, M. (2014). An overview on single nucleotide polymorphism studies in mastitis research. Vet World, 7(6):416-421.
• Kumar, A., Asaf V.N.M., Panigrahi, M., Ranjan, S., Bhushan, B. and Gaur, G.K. (2014). Single Nucleotide Polymorphism (SNP) in relation to mastitis in crossbred cattle. Ind Vet J, 91(10):50-52.
• Kumar, A., Asaf V.N.M., Raut, A.A., Sood, R. and Mishra, A. (2014). Identification of chicken pulmonary miRNAs targeting pb1, pb1-f2, and n40 genes of highly pathogenic avian influenza virus H5N1 in silico. Bioinformatics and Biology Insights 8:135-145. doi:10.4137/BBI.S14631
• Kumar, A., Asaf, V.N.M., Srivastava, K., Rahim, A., Chaudhary, J.K and Panigrahi, M. (2013). Microrna: biogenesis and computational target identification. Vet World, 6(10):761-765.
• Ramya, R., Sivanarayanan, T.B., Shebeeba, C.S., Sreelakshmi, C.M., Asaf, V.N.M., Sarangom, S.B. and Pillai, U.N. (2009). Juvenile Cellulitis in a Great Dane Pup. Intas Polivet, 10:379-380.

FULL PAPER IN CONFERENCES

• Desai, A.G., Thomas, N., Aravindakshan, T.V., Muhasin, V.N.A. and Lakshmanan, B. (2019). Characterization of a 7 bp indel in march1 promoter associated with birth weight of kids in native goat breeds of kerala. Proceedings of the 11^th Kerala Veterinary Science Congress, Kerala Veterinary and Animal Sciences University, 9-10 November 2019.
• Apurva, S.S., Sonia, K.R., Jais, A., Sankaralingam, S., Anitha, P., Priya, M., Mohan, K.M.S., Bindu, K.A. and Asaf, V.N.M. Identification of β-casein variants in cow milk. Proceedings of the 11^th Kerala Veterinary Science Congress, Kerala Veterinary and Animal Sciences University, 9-10 November 2019.

CONFERENCE ABSTRACTS

• Bora, A.F.C., Varghese, A., Nandini, A., Deepa, C.K., Lanchalung, M., Amrutha, B.M., Kurbet, P.S., Kumar, A.K.G., Ravindran, R., John, L. and Asaf, M. (2019). Molecular diagnosis of babesia gibsoni infection in dogs from northern zone of kerala. Proceedings of the 11^th Kerala Veterinary Science Congress, Kerala Veterinary and Animal Sciences University, 9-10 November 2019.
• Kamble, B.M., Dinesh, C.N., Ravindran, R., Rojan, P.M., Bindu, K.A., Asaf, M., Sreeshma, T.R., Deepna, T., Megnath, K.V. and Abdulkhader, A. (2019). Analysis of polymorphism in a QTL associated with tick resistance/susceptibility in cattle. Proceedings of the 11^th Kerala Veterinary Science Congress, Kerala Veterinary and Animal Sciences University, 9-10 November 2019.
• Thomas, N., Asaf, M. and Kurian, E. (2018). Tissue mRNA distribution and expression levels of rbp4 gene in prolific malabari and low prolific attappady black goats. Proceedings of Annual Conference on Propelling Transition towards Sustainable Food Production through Rekindling Physiological Strategies for addressing Contemporary Challenges, ICAR-Indian Veterinary Research Institute, 22 - 23 December 2018
• Asaf, M., Bhushan, B., Panigrahi, M., Kumar, A., Gupta, J.P., Ranjan, S., Kumar, A., Kumar, P. and Gaur, G.K. (2017). Differential expression profiling of il1b gene in host peripheral blood mononuclear cells (pbmcs) from mastitis tolerant and susceptible animals. Proceedings of the National Symposium on Biodynamic Animal Farming for the Management of Livestock Diversity under Changing Global Scenario, College of Veterinary and Animal Sciences, Mannuthy, 08-10 February 2017
• Asaf, V.N.M., Kumar, A., Raut, A.A. and Mishra, A. (2016). Validation of pulmonary miRNAs upregulated in chicken during highly pathogenic avian influenza infection & functional annotation of their target genes. Proceedings of the International Conference on RNAi 2016: RNA Biology and Therapeutics, University of Oxford, 05 April 2016
• Asaf, M., Bhushan, B., Panigrahi, M., Ranjan, S., Gupta, J.P. and Kumar, A. (2016). Statistical Analysis of single nucleotide polymorphisms located on MBL1 gene with somatic cell score reveals no significant association in vrindavani crossbred Cattle. Proceedings of the National Symposium on Policy Planning for Livelihood Security through Domestic Animal Biodiversity, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 11-12 February 2016
• Asaf, M., Bhushan, B., Panigrahi, M., Ranjan, S., Gupta, J.P. and Kumar, A. (2016). Association study of different single nucleotide polymorphisms located on NOD2 gene with mastitis in Tharparkar Cattle. Proceedings of the National Symposium on Policy Planning for Livelihood Security through Domestic Animal Biodiversity, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 11-12 February 2016
• Gupta, J.P., Bhushan, B., Asaf, M., Kumar, A., Panigrahi, M., Sulabh, S., Kumar, A.  and Sharma, D. (2016) . In Silico analysis and expression profiling of genotypes of a non-synonymous SNP observed in bovine CD14 gene. Proceedings of the National Symposium on Policy Planning for Livelihood Security through Domestic Animal Biodiversity, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 11-12 February 2016
• Asaf, V.N.M., Bhushan, B., Panigrahi, M., Gupta, J.P., Kumar, A., Ranjan, S., Sulabh, S., Kumar, P.  and Sharma, D. (2015). Association of single nucleotide polymorphism in the lactoferrin gene with mastitis in Vrindavani crossbred cattle.  Proceedings of International Symposium on Sustainable Management of Animal Genetic Resources for livelihood security in Developing Countries, Tamil Nadu Veterinary and Animal Sciences University, 13-14 February 2015.
• Asaf, V.N.M., Bhushan, B., Panigrahi, M., Gupta, J.P., Kumar, A., Ranjan, S., Sulabh, S., Kumar, P. and Sharma, D. (2015). Identifcation of single nucleotide polymorphisms in bovine CARD15 gene and their association with somatic cell score in vrindavani crossbred cattle. Proceedings of International Symposium on Sustainable Management of Animal Genetic Resources for livelihood security in Developing Countries, Tamil Nadu Veterinary and Animal Sciences University, 13-14 February 2015.
• Asaf, V.N.M., Kumar, A., Panigrahi, M., Ranjan, S., Dewangan, P., Bhushan, B. and Gaur, G.K. (2014). Association study of rs110326717 with mastitis tolerance in vrindavani Cattle. Proceedings of the National Symposium on Harmonizing Phenomics and Genomics for Sustainable Management of Livestock for Upliftment of Rural Masses, ICAR-National Bureau of Animal Genetic Resources, 06-07 February 2014
• Kumar, A., Asaf V.N.M.,  Panigrahi, M., Ranjan, S., Dewangan, P., Bhushan, B. and Gaur, G.K. (2014). Single nucleotide polymorphism (SNP) in relation to mastitis in Vrindavani Crossbred Cattle. Proceedings of the National Symposium on Harmonizing Phenomics and Genomics for Sustainable Management of Livestock for Upliftment of Rural Masses, ICAR-National Bureau of Animal Genetic Resources, 06-07 February 2014
• Asaf, V.N.M., Bhushan, B., Panigrahi, M., Gupta, J.P., Kumar, A., Ranjan, S., Kumar, P. and Sharma, D. (2014). Analysis of single nucleotide polymorphism at locus A526745G on CACNA2D1 gene with mastitis in vrindavani crossbred cattle. Proceedings of the National Seminar on Harmony with Nature in Context of Environmental Issues and Challenges of 21^st Century, Mohanlal Sukhadia University, 28-30 November 2014
• Mishra, A., Asaf, M., Kumar, A. and Raut, A.A. (2013). Understanding host response to highly pathogenic avian influenza (HPAIV) - a bioinformatics approach. Proceedings of the 3^rd IFIP International Conference on Bioinformatics, Maulana Azad National Institute of Technology, 23-26 September 2013

CHAPTERS IN TRAINING MANUALS

• Panigrahi, M., Sulabh, S., Gupta, J.P., Kumar, A., Asaf V.N.M. and Bhushan, B. (2015). Overview on breeds of buffaloes, cattle, goat, pigs and their characteristics and utility. In Animal Disease Control and Management, ICAR-Indian Veterinary Research Institute, Izatnagar.
• Panigrahi, M., Bhushan, B., Parida, S., Dewangan,P., Asaf V.N.M. and Kumar, A. (2014). Real time PCR: principle and chemistry. In Recent Advances in Transgenesis in Livestock, Indian Veterinary Research Institute, Izatnagar.
• Bhushan, B., Panigrahi, M., Dewangan, P., Kumar, R., Asaf, V.N.M., Kumar, A. and Parida, S. (2014). Total RNA isolation from tissue and cDNA synthesis. In Recent Advances in Transgenesis in Livestock, Indian Veterinary Research Institute, Izatnagar.
• Panigrahi, M., Bhushan, B., Dewangan, P., Kumar, R., Asaf, V.N.M., Kumar, A. and Parida, S. (2014). Protocol for Real time PCR using SYBR green. In Recent Advances in Transgenesis in Livestock, Indian Veterinary Research Institute, Izatnagar.

NON-REFERRED PUBLICATIONS

• Sebastian, R., Mohan, V., Asaf, V.N.M., Saravanan, S., Elaiyaraja, G. and Chabukdhara, P. (2014). Probiotics: A Promising way to boost your immune system. Dairy Standard, Pages 14-15.
• Mohan, V., Sebastian, R., Asaf V.N.M., Hari, R. and Reddy, A. (2014). Antimicrobials in milk and its public health significance. Dairy Standard, Pages16-18.
• Kumar, A., Asaf V.N.M., Panigrahi, M., Chaudhary, J.K., Shukla, A., Kumar, S., Amit, and Dewangan, P. 2014. Livestock for sustainable nutritional security of India. Livestock Line, 8(7): 44-46.
• Kumar, A., Kumar, R., Ranaware, P., Asaf, M., Kamboj, A. and Gupta, J.P. (2013). MicroRNA and Target Gene Prediction: An Overview. Himachal Veterinary Journal, 6:28-40.
• Asaf, M.,  Ansari, M.M., Kumar, A., Naheef, K., Kutty, M., Mohan, V. and Kumar, P. (2012). MicroRNA a tiny molecule with numerous functions: a review.  Livestock Line, 6(8): 6-8.

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